Free Biotechnology Principles And Processes (Weekly Quiz: 2) NEET Biology Questions & Answers with Detailed Explanation & Full Review

Question-1

In agarose gel electrophoresis

(a) larger molecules migrate faster than smaller molecules.

(b) supercoiled plasmids migrate slower than their nicked counterparts.

(c) DNA migrates towards the negative electrode.

(d) ethidium bromide can be used to visualize the DNA.

Question-2

Electrophoresis and Southern blotting techniques are used in

(a) Gene synthesis

(b) DNA fingerprinting

(c) Gene cloning

(d) All of these

Question-3

Which one of the following techniques made it possible to genetically engineer living organisms?

(a) Heavier isotope labelling

(b) X-ray diffraction

(c) Recombinant DNA techniques

(d) Hybridization

Question-4

There is a restriction endonuclease called Eco RI. What does 'co' part in it stands for?

(a) Strain of bacterium

(b) Coelom

(c) coli

(d) Colon

Question-5

Which method is suitable for transferring an alien DNA into a plant cell?

(a) Biolistics or gene gun

(b) $CaCl_2$

(c) Micro-infection

(d) Heat shock

Question-6

Which statement is incorrect ?

1. Retroviruses have also been disarmed and are now used to deliver desirable genes into animals cells.
2. Downstream processing is one of the steps of R-DNA technology.
3. DNA is a negatively charged molecule.
4. The presence of chromogenic substrate gives blue colour colonies, if the plasmid in the bacteria does not have an insert.

(a) I, III and IV

(b) I and II

(c) All of these

(d) None of these

Question-7

Which of the following enzymes catalyses the removal of nucleotides from the ends of DNA?

(a) exonuclease

(b) endonuclease

(c) DNA ligase

(d) Hind II

Question-8

Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?

(a) A stirred-tank bioreactor without inlets and outlets

(b) Laboratory flask of largest capacity

(c) A continuous culture system

(d) Any of the above

Question-9

Which of the following methods(s) is used to introduce foreign DNA into plant host cells?

(a) Gel electrophoresis

(b) Gene gun method

(c) Elution

(d) Extension

Question-10

Choose the incorrect statement.

(a) If a foreign DNA ligates at the Bam HI site of tetracycline resistance gene in the vector pBR322, the recombinant plasmid loses the tetracycline resistance due to insertion of foreign DNA

(b) Ori also controls the copy numbers of the linked DNA

(c) Copy number refers to the number of copies of plasmid present in a cell

(d) Copy number of plasmid varies from 50-100 per cell