Free Biotechnology Principles And Processes (Weekly Quiz: 1) NEET Biology Questions & Answers with Detailed Explanation & Full Review

Free Biotechnology Principles And Processes (Weekly Quiz: 1) Time:

Question-1

Restriction endonuclease - Hind II always cuts DNA molecules at a particular point by recognizing a specific sequence of

(a)

(b)

(c)

(d)

Explanation:

Answer: (c)

Restriction endonuclease-Hind II, always cuts DNA molecules at a particular point by recognizing a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II.


Question-2

The DNA molecules to which the gene of interest is integrated for cloning is called :

(a)

(b)

(c)

(d)


Question-3

DNA fragments are

(a)

(b)

(c)

(d)


Question-4

The below figure represents

biotechnology-principles-and-processes-neet-mcq-8-33-85

(a)

(b)

(c)

(d)


Question-5

The given figure shows the E.Coli cloning vector PBR322 showing restriction sites. Some parts are labelled as A, B, C & D. Choose the option showing the correct labelling.

biotechnology-principles-and-processes-class-12-Chapter-11-neet-mcq-quiz-pdf

 ABCD
(a)Hind IEcoR I$amp^{R}$ori
(b)Hind IBamH I$kan^{R}$$amp^{R}$
(c)BamH IPst I ori $amp^{R}$
(d)EcoR IBamH I$amp^{R}$ori

(a)

(b)

(c)

(d)

Explanation:

Answer: (d)

A-Eco RI; B-Bam HI; C-$amp^{R}$; D-ori. The given figure represents a physical map of plasmid pBR322. It is the first artificial and most widely used cloning vector. pBR322 is constructed from the plasmids of E.coli, pBR 318 and pBR 320. It contains origin of replication (Ori), antibiotic resistance genes e.g., ampicillin ($amp^{r}$) and tetracycline ($tet^{r}$), and unique recognition sites for 20 restriction endonucleases. Hind III, Eco R I, Bam H, Sal I, Pvu II, Pst I, Cla I are some restriction sites. Rop codes for the proteins involved in the replication of the plasmid.


Question-6

The construction of the first recombinant DNA was done by using the native plasmid of

(a)

(b)

(c)

(d)


Question-7

Number of bacterial strains from which restriction enzymes have been isolated.

(a)

(b)

(c)

(d)


Question-8

The recognition site for Bam HI in pBR322 is present in

(a)

(b)

(c)

(d)


Question-9

The primary reason why the same basic techniques can be used to analyze the DNA from species as diverse as bacteria and humans is that

(a)

(b)

(c)

(d)

Explanation:

Answer: (d)

The fact that DNA is structured the same way in all known organisms means that similar methods can be used to study the hereditary material.


Question-10

An antibiotic resistance gene in a vector usually helps in the selection of

(a)

(b)

(c)

(d)


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