Free Biotechnology Principles And Processes (Monthly Quiz: 1) NEET Biology Questions & Answers with Detailed Explanation & Full Review

Question-1

rDNA is a

(a) Hybrid DNA-RNA

(b) Chimeric DNA

(c) Recombinant of vector DNA and desired gene/s

(d) Both (b) and (c)

Question-2

DNA fragments are separated using gel electrophoresis

(a) to identify and isolate DNA fragments.

(b) because larger DNA fragments move faster through the gel than smaller DNA fragments.

(c) because DNA is pulled through the gel towards the negative end of the field.

(d) to synthesize DNA for cloning.

Question-3

In recombinant DNA technique, the term vector refers to a

(a) plasmid transfers DNA into host cell

(b) donor DNA, it is identified and picked up through electrophoresis

(c) collection of entire genome in the form of plasmid

(d) enzyme, cuts the DNA at specific sites

Question-4

Which of the following statement is incorrect ?

(a) When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of sticky ends.

(b) Each EcoRI restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.

(c) EcoRI cuts the DNA between bases G and A.

(d) Making multiple identical copies of any template DNA is called cloning.

Question-5

The bacteria generally used for genetic engineering is

(a) Pseudomonas

(b) Bacillus

(c) Agrobacterium

(d) Clostridium.

Question-6

In bacteria, plasmid is

(a) non-functional DNA

(b) main DNA

(c) extrachromosomal material

(d) repetetive gene

Question-7

The role of DNA ligase in the construction of a recombinant DNA molecule is

(a) formation of hydrogen bonds between sticky ends of DNA fragments

(b) formation of phosphodiester bond between two DNA fragments

(c) ligation of all purine and pyrimidine bases

(d) None of the above

Question-8

Assertion :
DNA is cleaved at specific site by all endonucleases.
Reason :
Endonucleases are found in viruses.

(a) If Assertion is true but Reason is false.

(b) If both Assertion and Reason are true but the Reason is not the correct explanation of the Assertion.

(c) If both Assertion and Reason are true and the Reason is the correct explanation of the Assertion.

(d) If both Assertion and Reason are false.

Question-9

In 'EcoRI' 'co' stands for

(a) Species

(b) Genus

(c) Strain

(d) Restriction enzyme

Question-10

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

(a) Negatively charged fragments do not move.

(b) Positively charged fragments move to farther end.

(c) The smaller the fragment size, the farther it moves.

(d) The larger the fragment size, the farther it moves.

Question-11

When the DNA fragments are observed under UV light, they are seen as

(a) orange coloured bands

(b) yellow coloured bands

(c) blue coloured bands

(d) Both (a) and (b)

Question-12

Assertion :
Restriction endonucleases have been designated as molecular scissors.
Reason :
Fragments produced by restriction endonucleases upon mixing join due to their sticky ends.

(a) If Assertion is true but Reason is false.

(b) If both Assertion and Reason are true but the Reason is not the correct explanation of the Assertion.

(c) If both Assertion and Reason are true and the Reason is the correct explanation of the Assertion.

(d) If both Assertion and Reason are false.

Question-13

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

(a) λ phage

(b) Ti plasmid

(c) Retrovirus

(d) pBR322

Question-14

In Ti-plasmid, which of the following is removed?

(a) Virulent gene

(b) Auxin gene

(c) Cytokinin gene

(d) Auxin and cytokinin gene

Question-15

Match the following columns.

Column I	Column II
A. Bacterial cell is treated with	1. Lysozyme
B. Plant cell is treated with	2. Cellulase
C. Fungal cell is treated with	3. Chitinase

Codes A B C

(a) 2 3 1

(b) 3 2 1

(c) 1 2 3

(d) 3 1 2

Question-16

Assertion:
Restriction endonucleases are also known as molecular scissors.
Reason:
It is used to cut DNA (Biomolecules) at specific site.

(a) If both the assertion and reason are true but the reason is not a correct explanation of the assertion.

(b) If both the assertion and the reason are true and the reason is a correct explanation of the assertion.

(c) If the assertion is true but the reason is false.

(d) If both the assertion and reason are false.

Question-17

DNA or RNA segment tagged with a radioactive molecule is called

(a) clone

(b) probe

(c) vector

(d) plasmid

Question-18

Two bacteria found to be very useful in genetic engineering experiments are

(a) Nitrosomonas and Klebsiella

(b) Rhizobium and Diplococcus

(c) Nitrobacter and Azotobacter

(d) Escherichia and Agrobacterium

Question-19

The most important feature in a plasmid to be used as a vector is

(a) presence of a selectable marker

(b) Origin of replication (Ori)

(c) presence of sites for restriction endonuclease

(d) its size

Question-20

Which is also called molecular glue?

(a) DNA helicase

(b) DNA gyrase

(c) DNA ligase

(d) DNA polymerase