FREE NEET Biology Practice MCQ Test: Biotechnology Principles And Processes Exercise 1 Questions Answers With Detailed Explanations [PDF]
Question : 1
Genes of interest can be selected from a genomic library by using
View Answer
Answer: (c)
A hybridization probe is a fragment of DNA of variable length which is used in DNA samples to detect the presence of nucleotide sequence (the DNA target) that are complementary to the sequence in the probe. The probe hybridize to single–stranded DNA whose base sequence allow probe target base-pairing due to complementary between the probe and target.
Question : 2
The figure below is the diagrammatic representation of the E. Coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?
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Answer: (d)
Question : 4
Commonly used vectors for human genome sequencing are:
View Answer
Answer: (a)
Human genome sequencing is a process that determines the complete DNA sequence of an organism’s genome at a single time. This requires sequencing all of an organism’s chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast. Commonly used vectors for human genome sequencing are BAC (Bacterial artificial chromosomes) and YAC(Yeast artificial chromosomes).
Question : 5
The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?
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Answer: (a)
Question : 6
The cutting of DNA at specific locations became possible with the discovery of :
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Answer: (b)
Question : 7
During the process of isolation of DNA, chilled ethanol is added to
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Answer: (a)
Ethanol is non-polar molecule while DNA is a polar molecule, it has a net negative charge due to the phosphate groups attached to it. When DNA is placed into a solution of 100% ethanol, it becomes insoluble and precipitates out of solution.
Question : 8
Which of the following antibiotic resistance genes are used as selectable marker for E. coli?
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Answer: (d)
Question : 9
The process of separation and purification of expressed protein before marketing is called:
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Answer: (b)
The various stages of processing that occur after the completion of fermentation or biosynthetic stage which include separation and purification of product called downstream processing.
Question : 10
- DNA being a hydrophilic molecule cannot pass through cell membranes.
- The bacteria should be made competent to accept the DNA molecule.
The correct option regarding the above statements is
View Answer
Answer: (c)
Question : 11
Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.
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Answer: (a)
The restriction endonuclease enzyme inspects the length of a DNA sequence. Once it recognises specific sequence, it binds to the DNA and cuts each of the two strands of the double helix at specific points in their sugar phosphate backbone. Special sequence in the DNA recognised by restriction endonuclease is called palindromic nucleotide sequence.
Question : 12
The taq polymerase enzyme is obtained from
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Answer: (b)
The Taq polymerase enzyme is obtained from Thermus aquaticus which lives in hot springs.
Question : 13
Which of the following bacteria is not a source of restriction endonuclease?
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Answer: (c)
Agrobacterium tumefaciens is not a source of restriction endonuclease but serves as the source of Ti plasmid.
On the other hand, Hind III is obtained from Haemophilius influenzae, Eco RI obtained from Escherichia coli and Bam HI obtained from Bacillus amyloliquefaciens.
Question : 14
Which one is a true statement regarding DNA polymerase used in PCR?
View Answer
Answer: (d)
Question : 15
A biologist intends to use a polymerase chain reaction to perform a genetic task. The biologist probably is trying to
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Answer: (b)
PCR is a way to increase the numbers of a particular sequence of DNA (or an entire gene). This technique is most useful when cloning DNA since prior knowledge of the DNA sequence must first be obtained.
Question : 17
Which of the following is true about EFB definition of biotechnology?
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Answer: (d)
Question : 18
Significance of the 'heat shock' method in bacterial transformation is to facilitate
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Answer: (c)
Question : 19
Identify the correct match for the given apparatus.
| Apparatus | Functions | |
| (a) | Gene gun | Vectorless direct gene transfer |
| (b) | Column chromatograph | Separation of chlorophyll pigments |
| (c) | Sparged tank bioreactor | Carry out fermentation process |
| (d) | Respirometer | Finding out rate of respiration |
View Answer
Answer: (c)
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NEET Biology MCQ
Topic Wise Exercise-
The Living World
-
Biological Classification
-
Plant Kingdom
-
Animal Kingdom
-
Morphology of Flowering Plants
-
Anatomy of Flowering Plants
-
Structural Organisation in Animals
-
Cell-The Unit of Life
-
Biomolecules
-
Cell Cycle and Cell Division
-
Transport in Plants
-
Mineral Nutrition
-
Photosynthesis in Higher Plants
-
Respiration in Plants
-
Plant Growth and Development
-
Digestion and Absorption
-
Breathing and Exchange of Gases
-
Body Fluids and Circulation
-
Excretory Products and Their Elimination
-
Locomotion and Movement
-
Neural Control and Coordination
-
Chemical Coordination and Integration
-
Reproduction In Organisms
-
Sexual Reproduction in Flowering Plants
-
Human Reproduction
-
Reproductive Health
-
Principles of Inheritance and Variation
-
Molecular Basis of Inheritance
-
Evolution
-
Human Health and Diseases
-
Strategies for Enhancement in Food Production
-
Microbes in Human Welfare
-
Biotechnology: Principles and Processes
-
Biotechnology and Its Applications
-
Organisms and Populations
-
Ecosystem
-
Biodiversity and Conservation
-
Environmental Issues
NEET Biology Quiz
Topic Wise Quizzes-
The Living World
-
Biological Classification
-
Plant Kingdom
-
Animal Kingdom
-
Morphology of Flowering Plants
-
Anatomy of Flowering Plants
-
Structural Organisation in Animals
-
Cell-The Unit of Life
-
Biomolecules
-
Cell Cycle and Cell Division
-
Transport in Plants
-
Mineral Nutrition
-
Photosynthesis in Higher Plants
-
Respiration in Plants
-
Plant Growth and Development
-
Digestion and Absorption
-
Breathing and Exchange of Gases
-
Body Fluids and Circulation
-
Excretory Products and Their Elimination
-
Locomotion and Movement
-
Neural Control and Coordination
-
Chemical Coordination and Integration
-
Reproduction In Organisms
-
Sexual Reproduction in Flowering Plants
-
Human Reproduction
-
Reproductive Health
-
Principles of Inheritance and Variation
-
Molecular Basis of Inheritance
-
Evolution
-
Human Health and Diseases
-
Strategies for Enhancement in Food Production
-
Microbes in Human Welfare
-
Biotechnology: Principles and Processes
-
Biotechnology and Its Applications
-
Organisms and Populations
-
Ecosystem
-
Biodiversity and Conservation
-
Environmental Issues
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