FREE NEET Biology Practice MCQ Test: Biotechnology And Its Applications Exercise 3 Questions Answers With Detailed Explanations [PDF]

Biotechnology And Its Applications Topic Based

Question : 1

Which of the following statements (i - v) is/are incorrect ?

  1. Recombinant DNA technology is used to improve crop plants by increasing their productivity, by making them more nutritious and by developing disease resistant.
  2. Bt cotton is resistant to bollworm infestation.
  3. Bacillus thuringiensis can form cry protein during any phase of their growth.
  4. Bacillus thuringiensis is not harmed by self cry protein because of its occurrence as protoxin (inactive).
  5. Protoxin cry protein is changed into active cry protein in the stomach of insects due to alkaline pH in stomach.


Answer: (c)

B thuringiensis forms protein crystals during a particular phase of their growth.

Question : 2

Assertion :
Blood clotting is prevented by Hirudin protein.
Reason :
The gene encoding for the Hirudin protein is transferred into Brassica napus, where accumulation of hirudin occurs in the seeds.


Answer: (a)

Hirudin protein prevents blood clotting. The gene that codes for this protein has been synthesized chemically and then transferred to Brassica napus for it to accumulate in the seeds.

Question : 3

A patient has a defective gene for the enzyme Adenosine Deaminase (ADA). He/She lacks functional cells and therefore, fails to fight the infecting pathogens. The cells are


Answer: (d)

The patient has a defective gene for the enzyme Adenosine Deaminase (ADA). This enzyme is involved in maturation of B and T-lymphocytes and is crucial for the functioning of immune system due to this, he/she lacks functional lymphocytes and therefore, fails to fight the infecting pathogens.

Question : 4

Read the following four statements (A-D) about certain mistakes in two of them.

  1. The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched.
  2. Restriction enzymes are used in isolation of DNA from other macromolecules.
  3. Downstream processing is one of the steps of rDNA technology.
  4. Disarmed pathogen vectors are also used in transfer of rDNA into the host.

Which of the two statements have mistakes?


Answer: (d)

In 1997, the first transgenic cow, Rosie, produced human protein enriched milk. The milk contained the human alpha-lactalbumin and was nutritionally a more balanced product for human babies than natural cow milk. Isolation of DNA from other macromolecules is achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).

Question : 5

The first human hormone produced by recombinant DNA technology is -


Answer: (c)

Question : 6

In RNAi, the genes are silenced using


Answer: (c)

Question : 7

Bt toxin protein crystals present in bacterium Bacillus thuringiensis, do not kill the bacteria because


Answer: (c)

Bt toxin protein crystals present in bacterium, Bacillus thuringiensis, do not kill the bacterium itself. This is because the toxin occurs as inactive protoxin in bacterium.

Question : 8

Which of the following Bt crops is being grown in India by the farmers?


Answer: (a)

Bt cotton is the first and only transgenic crop approved initially for cultivation by the farmers in India in six states namely, Andhra Pradesh, Gujarat, Karnataka, Madhya Pradesh, Maharashtra and Tamil Nadu.

Question : 9

RNA interference involves


Answer: (c)

RNAi is a method of cellular defense in all eukaryotes. It is a system within living cells that helps to control the activity of specific genes. This method involves silencing of mRNA due to complementary double stranded RNA that prevents translation of target gene or mRNA [silencing]. Source of ds RNA is retrovirus (having RNA genome) or transposons (mobile genetic material).

Question : 10

Human insulin is being commercially produced from a transgenic species of


Answer: (a)

Insulin is now being commercially produced by genetic engineering with the help of E.coli bacteria.

Question : 11

The first human hormone produced by recombinant DNA technology is


Answer: (b)

The first human hormone produced by recombinant DNA technology is insulin. It is a peptide hormone, which controls the level of glucose in blood.

Question : 12

GEAC stands for


Answer: (c)

GEAC stands for Genetic Engineering Approval Committee. The Indian government has set up this organisation to make decisions regarding the validity of GM research and the safety of introducing GM-organisms for public services.

Question : 13

The transgenic plants are the plants having


Answer: (d)

Question : 14

Bt corn has been made resistant to corn borer by the introduction of gene


Answer: (c)

Question : 15

α–1 Antitrypsin is


Answer: (d)

α- antitrypsin is a protease inhibitor which is produced by the introduction of specific sequence of DNA or genes that codes for a particular product in the transgenic animals. It is used to treat emphysema as it inhibits the enzymes of inflammatory cells that contributes to the respiratory complications.

Question : 16

What is the source of $T_i$ plasmid which is modified and used as a cloning vector to deliver the desired genes into plant cells?


Answer: (b)

Question : 17

RNAi stands for


Answer: (c)

Question : 18

The protein a-1 antitrypsin is used to treat the


Answer: (d)

Emphysema is a condition in which the air sacs of the lungs are damaged and enlarged, causing breathlessness. The protein alpha - 1 antitrypsin is used to treat the emphysema. Alpha - 1 antitrypsin is a protein made by cells in the liver and passes out into the bloodstream and can travel to the lungs. Its main function is to protect the lungs from damage caused by other types of proteins.

Question : 19

Genetically modified food is not desirable because


Answer: (d)

Question : 20

Which of the following statement(s) is/are correct?


Answer: (d)

All the statements are correct.

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