Free Biotechnology Principles And Processes (Daily Quiz: 2) NEET Biology Questions & Answers with Detailed Explanation & Full Review

Question-1

Eukaryotic genes do not function properly when cloned into a bacterial cell, because

(a) of inability to excise introns and destruction by bacterial restriction enzymes

(b) of high pH present in bacterial cells

(c) of inappropriate insertion of genes

(d) both (a) and (b).

Question-2

Select the incorrect statement.

(a) In the year 1963, the two enzymes responsible for restricting the growth of bacteriophage in Escherichia coli were isolated.

(b) More than 900 restriction enzymes have been isolated from over 230 strains of bacteria.

(c) Some key tools for recombinant DNA technology are restriction enzyme, polymerase enzyme, ligase, vectors and host root organisms.

(d) EcoRI cut the DNA between bases A and T only when the sequence GAATTC is present in the DNA.

Question-3

A gene whose expression helps to identify transformed cell is known as

(a) structural gene

(b) plasmid

(c) vector

(d) selectable marker.

Question-4

In gel electrophoresis, the separated DNA fragments are visualised after staining the DNA with EtBr followed by exposure to …. . Choose the appropriate option.

(a) UV-radiation

(b) Infrared radiation

(c) γ-rays

(d) Radiowave

Question-5

EcoRI cuts palindrome sequence which produces overhanging stretches called sticky ends on each strand. These are named sticky because

(a) They form hydrogen bonds with their complementary cut counterpart.

(b) They can combine with any DNA.

(c) They facilitate the action of the enzyme DNA ligase.

(d) Both (a) and (c)