Free Biotechnology Principles And Processes (Daily Quiz: 11) NEET Biology Questions & Answers with Detailed Explanation & Full Review

Free Biotechnology Principles And Processes (Daily Quiz: 11) Time:

Question-1

DNA ligases are enzymes that can be used to

(a)

(b)

(c)

(d)

Explanation:

Answer: (c)

Ligases are used to connect short DNA fragments to form longer segments, a process essential for inserting DNA segments into vectors.


Question-2

Restriction endonuclease

(a)

(b)

(c)

(d)


Question-3

The figure below is the diagrammatic representation of the E.coli vector pBR 322. Which one of the given options correctly identifies its certain component's) ?

biotechnology-principles-and-processes-class-12-Chapter-11-neet-mcq-9-236-29

(a)

(b)

(c)

(d)


Question-4

In gel electrophoresis, restriction enzyme digested DNA is loaded in wells near

(a)

(b)

(c)

(d)

Explanation:

Answer: (a)

DNA being negatively charged migrates towards the positive terminal, the anode, but is loaded at cathode.


Question-5

Which of the following is a restriction endonuclease?

(a)

(b)

(c)

(d)

Explanation:

Answer: (a)

Hind II is the first restriction endonuclease. It was isolated from Haemophilus influenzae Rd. It always cut DNA at specific position producing blunt ends. DNase I is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide non-specially. RNase is a type of nuclease that catalyses the degradation of RNA into smaller components. It can be endoribonuclease or exoribonuclease. A protease is an enzyme that perform proteolysis, i.e., protein catabolism by hydrolysis of the peptide bonds.


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