Free Biotechnology Principles And Processes (Daily Quiz: 9) NEET Biology Questions & Answers with Detailed Explanation & Full Review

Question-1

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

(a) 5'-GATATG-3'
3'–CTACTA–5'

(b) 5'–CGTTCG –3'
3'–ATGGTA–5'

(c) 5'–GAATTC-3'
3'–CTTAAG–5'

(d) 5'–CACGTA–3'
3'–CTCAGT– 5'

Question-2

Match the Column I with Column II and choose the correct option.

Column I	Column II
A. Southern blotting	1. Running of DNA fragments on gel
Gel electrophoresis	2. Cutting of DNA into fragments
Cleavage	3. Transfer of DNA fragment from gel to nitrocellulose disc
DNA probing	4. Searching for desired DNA fragments

(a) A-(2); B-(1); C-(4); D-(3)

(b) A-(4); B-(1); C-(2); D-(3)

(c) A-(4); B-(1); C-(3); D-(2)

(d) A-(1); B-(4); C-(2); D-(3)

Question-3

The DNA fragments separated on an agarose gel can be visualised after staining with

(a) ethidium bromide

(b) aniline blue

(c) acetocarmine

(d) bromophenol blue.

Question-4

Which of the following bacteria is not a source of restriction endonuclease?

(a) Escherichia coli

(b) Haemophilus influenzae

(c) Agrobacterium tumefaciens

(d) Bacillus amylo

Question-5

30 cycles of PCR amplified DNA approximately is how many times?

(a) 1 million times

(b) 1 billion times

(c) 100 times

(d) 1000 times