Free Biotechnology Principles And Processes (Daily Quiz: 6) NEET Biology Questions & Answers with Detailed Explanation & Full Review

Free Biotechnology Principles And Processes (Daily Quiz: 6) Time:

Question-1

The DNA molecule to which the gene of interest is integrated for cloning is called:

(a)

(b)

(c)

(d)


Question-2

Two microbes found to be very useful in genetic engineering are

(a)

(b)

(c)

(d)

Explanation:

Answer: (d)

Escherichia coli is a bacterium found in human colon. On this bacterium scientists have made extensive genetic experiments to make some vital chemicals like insulin. Another bacterium is Agrobacterium tumefaciens which causes crown gall in plants is extensively used for genetic experiments.


Question-3

Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?

(a)

(b)

(c)

(d)

Explanation:

Answer: (d)

Agarose gel electrophoresis does not provide a means of visualizing the DNA but that role is played by two dyes: ethidium bromide and loading buffer. Ethidium bromide, known as an intercalating agent allows it to intercalate, or insert, between nitrogenous bases of a DNA molecule. When it is exposed to ultraviolet light, ethidium bromide fluoresces. Thus, this chemical provides both a means of tagging DNA molecules and a means of visualizing them.


Question-4

Assertion:
In EcoRI, the latter R is derived from the genus of bacteria.
Reason:
EcoRI is the name of palindromic nucleotide sequences.

(a)

(b)

(c)

(d)


Question-5

The DNA fragments separated on an agarose gel can be visualised after staining with :

(a)

(b)

(c)

(d)

Explanation:

Answer: (c)

Ethidium bromide (Et Br) is used to stain the DNA fragments and will appear as orange coloured bands when kept under UV light.


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